GSE161693: Notch2 deficiency prevents muscle atrophy induced by mechanical unloading and diabetes.

Gene Expression Data Explorer
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GROUP CONDITION SAMPLES
Gastrocnemius muscle
GSM4912804 GSM4912805 GSM4912806 GSM4912807 GSM4912816 GSM4912817 GSM4912818 GSM4912819
GSM4912800 GSM4912801 GSM4912802 GSM4912803 GSM4912812 GSM4912813 GSM4912814 GSM4912815
GSM4912796 GSM4912797 GSM4912798 GSM4912799 GSM4912808 GSM4912809 GSM4912810 GSM4912811
Description

Submission Date: Nov 18, 2020

Summary: Notch signaling plays essential roles in maintenance of muscle stem cell pool. We found that Notch2, but not Notch1 and Notch3, is expressed in fully differentiated myofibers. To study the specific role of Notch2 in adult myofibers, we generated muscle-specific Notch2-knockout mice. Here, we showed that muscle-specific Notch2 deficiency prevented muscle atrophy induced by hindlimb unloading and diabetes millitus. RNA sequencing analysis revealed that the loss of Notch2 gene in myofibers inhibited gene responses to unloading and diabetes. Especially, several FoxO-target genes and atrogenes were upregulated in wildtype muscles but not in Notch2-deficient muscles by unloading and diabetes. Thus, our characterization of muscle-specific Notch2-knockout mice indicates that Notch2 acts as a regulatory factor of skeletal muscle plasticity and could be a therapeutic target of muscle atrophy.

GEO Accession ID: GSE161693

PMID: 35228746

Description

Submission Date: Nov 18, 2020

Summary: Notch signaling plays essential roles in maintenance of muscle stem cell pool. We found that Notch2, but not Notch1 and Notch3, is expressed in fully differentiated myofibers. To study the specific role of Notch2 in adult myofibers, we generated muscle-specific Notch2-knockout mice. Here, we showed that muscle-specific Notch2 deficiency prevented muscle atrophy induced by hindlimb unloading and diabetes millitus. RNA sequencing analysis revealed that the loss of Notch2 gene in myofibers inhibited gene responses to unloading and diabetes. Especially, several FoxO-target genes and atrogenes were upregulated in wildtype muscles but not in Notch2-deficient muscles by unloading and diabetes. Thus, our characterization of muscle-specific Notch2-knockout mice indicates that Notch2 acts as a regulatory factor of skeletal muscle plasticity and could be a therapeutic target of muscle atrophy.

GEO Accession ID: GSE161693

PMID: 35228746

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Bulk RNA-seq Appyter

This pipeline enables you to analyze and visualize your bulk RNA sequencing datasets with an array of downstream analysis and visualization tools. The pipeline includes: PCA analysis, Clustergrammer interactive heatmap, library size analysis, differential gene expression analysis, enrichment analysis, and L1000 small molecule search.