GSE157585: Metformin Alters Skeletal Muscle Transcriptome Adaptations to Resistance Training in Older Adults

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GROUP CONDITION SAMPLES
Vastus lateralis muscle
GSM4771046 GSM4771047 GSM4771048 GSM4771049 GSM4771050 GSM4771051 GSM4771052 GSM4771055 GSM4771056 GSM4771057 GSM4771062 GSM4771063 GSM4771064 GSM4771065 GSM4771066 GSM4771067 GSM4771068 GSM4771073 GSM4771074 GSM4771075 GSM4771078 GSM4771079 GSM4771080 GSM4771081 GSM4771084 GSM4771087 GSM4771091 GSM4771093 GSM4771094 GSM4771095 GSM4771096 GSM4771097 GSM4771098 GSM4771101 GSM4771106 GSM4771107 GSM4771110 GSM4771111 GSM4771112 GSM4771113 GSM4771114 GSM4771117 GSM4771121 GSM4771122 GSM4771123 GSM4771124 GSM4771125 GSM4771133 GSM4771138 GSM4771139 GSM4771140 GSM4771141 GSM4771142 GSM4771143 GSM4771146 GSM4771147 GSM4771152 GSM4771153 GSM4771154 GSM4771155 GSM4771156 GSM4771157
GSM4771161 GSM4771162 GSM4771163 GSM4771164 GSM4771165 GSM4771166 GSM4771167 GSM4771168 GSM4771169 GSM4771170 GSM4771171 GSM4771172 GSM4771173 GSM4771174 GSM4771175 GSM4771177 GSM4771178 GSM4771179 GSM4771180 GSM4771181
GSM4771053 GSM4771054 GSM4771058 GSM4771059 GSM4771060 GSM4771061 GSM4771069 GSM4771070 GSM4771071 GSM4771072 GSM4771076 GSM4771077 GSM4771082 GSM4771083 GSM4771085 GSM4771086 GSM4771088 GSM4771089 GSM4771090 GSM4771099 GSM4771100 GSM4771102 GSM4771103 GSM4771104 GSM4771105 GSM4771109 GSM4771115 GSM4771116 GSM4771118 GSM4771119 GSM4771120 GSM4771126 GSM4771127 GSM4771128 GSM4771129 GSM4771130 GSM4771131 GSM4771132 GSM4771134 GSM4771135 GSM4771136 GSM4771137 GSM4771144 GSM4771145 GSM4771148 GSM4771149 GSM4771150 GSM4771151 GSM4771158 GSM4771159 GSM4771160
Description

Submission Date: Sep 07, 2020

Summary: Evidence from clinical trials and observational studies suggests that both progressive resistance training (PRT) and metformin delay a variety of age-related morbidities. Previously, we completed a clinical trial testing the effects of 14 weeks of PRT + metformin (metPRT) compared to PRT with placebo (plaPRT) on muscle hypertrophy in older adults. We found that metformin blunted PRT-induced muscle hypertrophic response. To understand potential mechanisms underlying the inhibitory effect of metformin on PRT, we analyzed the muscle transcriptome in 23 metPRT and 24 plaPRT participants. PRT significantly increased expression of genes involved in extracellular matrix remodeling pathways, and downregulated RNA processing pathways in both groups, however, metformin blunted the number of differentially expressed genes within these pathways compared to plaPRT. Pathway analysis showed that genes unique to metPRT modulated aging-relevant pathways, such as cellular senescence and autophagy. Differentially expressed genes from baseline biopsies in older adults compared to resting muscle from young volunteers were reduced following PRT in plaPRT and were further reduced in metPRT. We suggest that although metformin may blunt pathways induced by PRT to promote muscle hypertrophy, adjunctive metformin during PRT may have beneficial effects on aging-associated pathways in muscle from older adults.

GEO Accession ID: GSE157585

PMID: 33071237

Description

Submission Date: Sep 07, 2020

Summary: Evidence from clinical trials and observational studies suggests that both progressive resistance training (PRT) and metformin delay a variety of age-related morbidities. Previously, we completed a clinical trial testing the effects of 14 weeks of PRT + metformin (metPRT) compared to PRT with placebo (plaPRT) on muscle hypertrophy in older adults. We found that metformin blunted PRT-induced muscle hypertrophic response. To understand potential mechanisms underlying the inhibitory effect of metformin on PRT, we analyzed the muscle transcriptome in 23 metPRT and 24 plaPRT participants. PRT significantly increased expression of genes involved in extracellular matrix remodeling pathways, and downregulated RNA processing pathways in both groups, however, metformin blunted the number of differentially expressed genes within these pathways compared to plaPRT. Pathway analysis showed that genes unique to metPRT modulated aging-relevant pathways, such as cellular senescence and autophagy. Differentially expressed genes from baseline biopsies in older adults compared to resting muscle from young volunteers were reduced following PRT in plaPRT and were further reduced in metPRT. We suggest that although metformin may blunt pathways induced by PRT to promote muscle hypertrophy, adjunctive metformin during PRT may have beneficial effects on aging-associated pathways in muscle from older adults.

GEO Accession ID: GSE157585

PMID: 33071237

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