Select conditions below to toggle them from the plot:
GROUP | CONDITION | SAMPLES |
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Whole blood |
GSM3509074 GSM3509075 GSM3509077 GSM3509078 GSM3509079 GSM3509080 GSM3509081 GSM3509082 GSM3509084 GSM3509085 GSM3509087 GSM3509089 GSM3509090 GSM3509091 GSM3509092 GSM3509093 GSM3509094 GSM3509095 GSM3509096 GSM3509097 GSM3509098 GSM3509099 GSM3509100 GSM3509101 GSM3509103 GSM3509104 GSM3509106 GSM3509107 GSM3509108 GSM3509109 GSM3509110 GSM3509111 GSM3509112
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GSM3509038 GSM3509044 GSM3509045 GSM3509046 GSM3509051 GSM3509052 GSM3509053 GSM3509055 GSM3509058 GSM3509060 GSM3509065 GSM3509068 GSM3509071 GSM3509073
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Submission Date: Dec 11, 2018
Summary: Patients affected by type 1 diabetes are recruited in the departments of Diabetology and Healthy Volunteers (HV) are selected based on internal records in the same hospital. Total RNA from whole blood has been extracted following a two-step procedure. First, RNA from blood collected on PAX-Gene tubes has been extracted using Maxwell 16 LEV simplyRNA blood kit (Promega) following manufacturer recommendations and second b-globin, dominant RNA from red blood cells, has been removed using the GLOBINclear kit (Ambion) on extracted RNA. RNA sequencing has been performed from using the TruSeq Stranded mRNA preparation kit (Illumina) on 500 ng b-globin depleted RNA with a RNA Integrity Number > 8 (measured on Bioanalyzer following manufacturer recommendations), and then sequenced following a pair-end 2x75 bp protocol on NextSeq 500 or HiSeq 4000 (Illumina) at LIGAN Equipex (Lille, France).
GEO Accession ID: GSE123658
PMID: No Pubmed ID
Submission Date: Dec 11, 2018
Summary: Patients affected by type 1 diabetes are recruited in the departments of Diabetology and Healthy Volunteers (HV) are selected based on internal records in the same hospital. Total RNA from whole blood has been extracted following a two-step procedure. First, RNA from blood collected on PAX-Gene tubes has been extracted using Maxwell 16 LEV simplyRNA blood kit (Promega) following manufacturer recommendations and second b-globin, dominant RNA from red blood cells, has been removed using the GLOBINclear kit (Ambion) on extracted RNA. RNA sequencing has been performed from using the TruSeq Stranded mRNA preparation kit (Illumina) on 500 ng b-globin depleted RNA with a RNA Integrity Number > 8 (measured on Bioanalyzer following manufacturer recommendations), and then sequenced following a pair-end 2x75 bp protocol on NextSeq 500 or HiSeq 4000 (Illumina) at LIGAN Equipex (Lille, France).
GEO Accession ID: GSE123658
PMID: No Pubmed ID
Signatures:
Control Condition
Perturbation Condition
Only conditions with at least 1 replicate are available to select
This pipeline enables you to analyze and visualize your bulk RNA sequencing datasets with an array of downstream analysis and visualization tools. The pipeline includes: PCA analysis, Clustergrammer interactive heatmap, library size analysis, differential gene expression analysis, enrichment analysis, and L1000 small molecule search.